To be a distinguished center in parasitological research, providing high quality research at both diagnostic and treatment levels
To provide a high quality of diagnostic laboratory service in an effective and timely manner in addition to develop new diagnostic techniques for endemic parasitic diseases
Head of the Laboratory
Prof. Ibrahim R. Aly Shalash
Former Heads And Staff Members
Prof. Maisa Ahmed Shalaby
Prof. Amal Mohamed Abdou
Prof. Fatem Ramzy Guirguis
Prof. Soheir Sayed Mohammed Mahmoud
Prof. Zeinab Hassanien Fahmy
Prof. Mohamed Abdel Gayid
Prof. Eman Aly Muostafa
Assistant.Prof. Wafaa Mohamed Abdel Fatah
Assistant.Prof. Eman Ahmed Hassan
Prof. Nevine Guirguis Nessim
Prof. Samia William Abdel Sayed
Prof. Tarek Mahmoud Diab
Ass.Prof. Gehan Labib Hafez
Ass.Prof. Azza Moustafa Fahmy
Ass.Prof. Rabab El-Sayed Mohamed
Ass. Lecturer: Shimaa Shaker
Ass. Lecturer: Hagar Fathy
Ass. Lecturer: Eman EL-Shabrawy
Ass. Lecturer: Shimaa Fouad
Ass. Lecturer: Mena El Rahman Ahmed Fahmy
Specialist: Reem Ahmed El Sheik
Specialist: May Abdel Wehab Mohamed
Specialist: Shaimaa Fathy Foud
Specialist: Pola Robil Michael
Specialist: Noran Abd El Nasser Al Brolos
Specialist: Maha Taalat El Hanafy Mahmoud
Field of Research
The parasitology department focuses on:
a.Implementation and update of diagnostic techniques in managing endemic parasitic infections and their complications.
b- Evaluation and monitoring of drug efficacies and emergence of resistance
c- Development of new candidate vaccines against parasites.
d- Implications of molecular techniques in medical parasitological studies.
e- Utilization of nanotechonology in various research fields.
a. Three innovative prototypes were submitted to the Egypian Patency Office, Academy of Scientfic Research and Technology:
• Web-based Diagnosis of parasitic diseases using I-Phone. Temporary patency is issued(submitted in 2016, no. 16). PI: Associate Prof. Gehan Labib Hafez, Associate Prof. Rabab Sayed Zalat and Prof. Dr. Ibrahim Aly
b. Identification, characterization and preparation of target antigens of different parasites of medical importance and using them in both diagnosis and treatment of these parasites.
c. Evaluation of newly introduced drugs and purified plant extracts as antiparasitic agents
d. Application of newly introduced molecular techniques in the diagnosis of endemic parasites
A. Routine urine and stool microscopic examinations using different parasitological techniques.
B. Introduction of special new techniques for stool analysis as parasep faecal concentration technique including
• Diagnosis and identification of different opportunistic protozoa in stool samples using different staining techniques including Zeihl Neelsen, Modified acid fast, Trichrome and modified trichrome as well as Safranine stains.
• Performing special staining methods or culture media for detection of newly emerging protozoa (D. fragilis & B. hominis) as Giemsa stain
• Applying special stains for detection of protozoa as Safranine Stain & Auramine Rhodamine Stains.
D. Field surveys using specific parasitological techniques including nucleopore filtration of urine for S. haematobium ova, Kato technique for different parasitic ova in stool and Negrosine technique for different parasitic ova in stool.
E. Diagnosis of different parasites and protozoa in stool samples using immunoparasitological assays (our lab prepared assays or newly introduced kits) including:-
• ELISA kits for detection of circulating parasitic antigens in patients' sera as well as copro-antigens in patients' stool Merifluor immunofluorescent stain.
• Applying a modified method for the direct wet mount preparation for better and clear identification of parasites.
F. Periodic parasitological examination of personnel and workers in different labs, especially those subjected to contamination with biological materials.
A. Standard experimental parasitological examinations to measure the intensity of schistosomal infection and morbidity including:
• Worm perfusion to count the number of worms present in liver and intestine of infected mice.
• Ova count in tissues (liver and intestine)
• Oogram pattern (counting different developmental stages of the parasites' ova in small intestine.
B. Identification, characterization and preparation of target antigens of different parasites of medical importance in large amounts using molecular biological tools and applying it in:-
• Development of simple, sensitive, less time consuming and cheap kits using nanotechnology for diagnosis of different multiple parasites (as ELISA plate, coated by different antigens) (screening test).
• Production of highly sensitive and specific antibodies against these target antigens using phage display technique and applying it in diagnostic kits.
• Development of candidate vaccines.
• Production of nanoparticles as a drug delivery system for targeting of parasitic infections.
• Development of a quick diagnostic strip kit for field surveys (running) from rabbits, on strip or ring, for the detection of Copro-antigens of different parasites (Schistosoma and Fasciola to start with..) could be applied commercially.
C. Tissue culture unit which is used in:-
• In vitro culture of parasites for immunolocalization studies
• Maintenance of life cycle of some parasites and protozoa (Schistosoma, Toxoplasma, Cryptospordium and Blastocystis) and production of target antigens and biological materials secreted from these parasites.
• Evaluation of antiparasitic efficacy of newly introduced drugs and plant extracts as well as classical drug treatment either alone or combined with other materials including anti-inflammatory, antifibrotic agents, natural and newly introduced synthetic compounds.
• Evaluation of different vaccines against parasites
Laboratory Structure and Facilities
a. Annual training program for laboratory personnels working on the diagnosis of parasitic diseases.
b. Two weeks rotation program in the outpatient parasitology lab for Faculty of Medicine and Science undergraduate students.
c. Annual teaching of parasitology course for TBRI nursing school students.
Scope of Services
The policy of the department is to analyze urine and stool samples of immuncompetent and immunocomprornised patients using different parasitological techniques for both inpatients and outpatients. Also, to perform monthly statistical analysis for data.
The scope of services offered by the department focus on:
a. Gastrointestinal diseases, urinary tract infections, Some blood stream parasites (e.g. toxoplasma), Fever of unknown origin, Post-surgical parasitic infections as well as infections related to compromised immunity in hepatic and chronic renal patients.
b. Monitoring of drug efficacy and emergence of drug resistance parasitic isolates (strains) including Schistosomiasis mansoni and hematobium, Fascioliasis (hepatica and gigantica) and Protozoa e.g., Entamebia species (histolytica and dispar).
Internal research projects:
Novel diagnostic and therapeutic tools in marine models of hepato cellar carcinoma a pilot study. PI: Prof. Eman El-Ahwany (2015)
Molecular and Immunohistochemical detection of Cryptosporidium-induced intestinal tissue alterations in dexamethasone treated &untreated mice.
· External Projects:
American University in Cairo: Nanoparticles as a delivery system for targeting Schistosomiasis and Fascioliasis: A pilot study using Nanotechnology and bioinformatics. PI: Prof. Suher Zada (2010)
El- Jameel center for Science and Technology & the American University in Cairo: Cloning expression of target antigen (Cathepsin L1&L2) from Schistosoma mansoni and Fasciola gigantica. PI: Prof. Suher Zada (2010)
Project STDF, ID 1454: Development of production and pegylation of interferon alpha. PI: Prof. Ehab El-Dabaa Mohammed Sabry (2010)
Egyptian and French Collaboration project (Lyon University): Development and evaluation of immunoblot and immunochromatography test kits for the diagnosis of helminthic infections in Egypt. PI: Prof. Ibrahim Rabie (2011)
Ministry of Scientific research: Nanotechnology based drug delivery for treatment of multi-drug resistant tuberculosis. PI: Prof. Mohammed Abbas Shemis (2011).
American University in Cairo: Treg cells, a new lymphocyte subpopulation with a key role in the immuno response against Schistosoma mansoni infection. PI: Prof. Suher Zada (2014)
A novel Nanotechnology based approach for the rapid diagnosis of infectious diseases using multiple nano- chip. PI: Prof. Ibrahim Rabie (2017)
Nagy FM, Rabia I, EL-Kersh WM (2010) Home-made monoclonal antibody–based sandwich ELISA versus commercial fast dot-ELISA technique in the diagnosis of human schistosomiasis and fascioliasis. NewYork Science Journal, 3:89-97
Rabia I, El-Ahwany E, El-Komy W, Nagy F (2010). Immunomodulation of Hepatic Morbidity in Murine Schistosomiasis mansoni Using Fatty Acid Binding Protein Journal of American Science, 6
Rabia I, Nagy F, Ali E, Mohamed A, El-Assal F, El-Amir A (2010). OL-037 Effect of treatment with antifibrotic drugs in combination with PZQ in immunized Schistosoma mansoni infected murine model. International Journal of Infectious Diseases, 14:S16-S17
SH IRM, Demerdash Z, Diab T, Maher K, Safwat W, Shaker Z (2010). Anti-S. mansoni mAb-based latex agglutination: a reliable field applicable immunodiagnostic test for screening of active human schistosomiasis. Journal of American Science, 6
Ramzy F, Mahmoud S, William S (2010). Further assessment of Mirazid as antischistosomal drug in experimental schistosomiasis hematobium. Pharmaceutical biology, 48:775-779
Kamel E, El-Emam M, Mahmoud S, Fouda F, Bayaumy F (2011). Parasitological and biochemical parameters in Schistosoma mansoni-infected mice treated with methanol extract from the plants Chenopodium ambrosioides, Conyza dioscorides and Sesbania sesban. Parasitology International, 60:388-392
Mahmood NM, Ramadan NF, Hassan SM, Sabry H, Magdy MM (2016). Therapeutic effect of Phoenix dactylifera against cryptosporidiosis in immunocompromised mice. Global Advanced Research Journal of Medical Sciences, 5:088-095
Khater MM, El-Sayed SH, Yousof H-AS, Mahmoud SS, El-Dib N, El-Badry AA (2017). Anti-Cryptosporidium efficacy of Olea europaea and Actinidia deliciosa in a neonatal mouse model. Kasr Al Ainy Medical Journal, 23:32
Mansy SS et al. (2017). Potential ultrastructure predicting factors for hepatocellular carcinoma in HCV infected patients. Ultrastructural Pathology 41:209-226
Sakran T, El-Shahawy G, Shalaby M, Sabry H, Matooq P, Elmallah A (2017). Detection rates of waterborne protozoa in water sources from Fayoum Governorate. Parasitologists United Journal 10:30-38
Aly I, Taher EE, Gehan E, Sayed HE, Mohammed FA, Hamad RS, Bayoumy EM (2018). Advantages of bioconjugated silica-coated nanoparticles as an innovative diagnosis for human toxoplasmosis. Acta tropica, 177:19-24