Vision


Central lab of TBRI aims to be a well recognized medical research center of excellency on both regional and African levels.

Mission


 Provide technical support and consultancy needed for qualified research activities in pursuit of innovation and leadership in research laboratory medicine.

Also, to optimize and upgrade the technical performance and experience of all researchers within TBRI as well as from other institutes and universities.

 

Staff Members


Head: Prof. Faiza Essawy Professor of Haematology

Prof. Iman Khaled Professor of Haematology

Prof. Abd Allah Morsy Professor of Clinical Chemistry

Prof. Hoda Abu Taleb Professor of Environmental Research

Assist. Prof. Doaa Gamal Assistant Professor of Microbiology

Specialist Rady El Araby

Specialist Fawzy Roshdy

Specialist Ismael Refky

Central Laboratory Structure


 •         Cell Culture & Microscopy Laboratory.

         Flow Cytometry & Serology Laboratory.

         Molecular Laboratory.

 

 Main Equipment and Devices

-FLOW CYTOMETRY AND SEROLOGY LABORATORY

 1-    Flow cytometry

 2-    ELISA Set

Reader + Washer + Microplate shakerincubator

 

 3-    Double Beam Spectrophotometer

Reads wavelength ranges from 190 to 900nm.

Gives better resolution and sensitivity.

 

-TISSUE CULTURE LABORATORY

 

1-    CO2 Incubator

2-    Laminar air flow class II A2

3-    Zeiss trinocular inverted microscope

4-    Zeiss trinocular light microscope

5-    Ambient Incubator

   6-    Zeiss digital camera and screen for microscopes allowing

Highlighting certain focus in a higher magnification.

Writing a text.

Measuring distances (in microns).

Counting certain objects.

Marking certain object (by arrow or X).

7-  Liquid nitrogen container

 

 

MOLECULAR LAB 

 

1- Nano-drop spectrophotometer: 

 

 

 

 

 

                                   

 

 

 

Microplate spectrophotometer

(6- to 384- well plate reading capability)

for low volume nucleic  acid quantification.

 

 

 

2- PCR Cabinet 

 

                                              

 

 

                

 

 

 

 

 

 

3- Laminar Flow Class II                    

 

 

 

4- Automated DNA/RNA extractor:             

 

 

 

 

 

 

 

 

 

*Using magnetic beads technology.

*Superior purity and yields compared to

other techniques.

*Reduce processing duration.

*Purify protein and nucleic acid from FFPE tissue,

whole blood, cells, or fresh and frozen tissues.

 

 

 

5- Roche Real Time PCR: 

                                               

 

 

 

 

 

 

 

*LightCycler® 480 Instrument is a plate-based real-time PCR device that supports mono-

or multicolor applications, as well as multiplex protocols.

*For qualitative and quantitative detection of nucleic acids.

*Wide range of probe formats e.g. FRET, florescent

dyes e.g. SYBR Green I and detection formats e.g.

high resolution melting (HRM); a new post-

PCR analysis method used to identify variations

in nucleic acid sequences.

 

 

6- Biometra Gradient Thermal cycler: 

                                  

 

 

 

 

 

 

 

*With block material made of silver coated with gold, giving best accuracy with superior sample block temperature control.

*Linear Gradient Tool: For easy gradient programming using simultaneously different primer annealing temperatures.

 

 

7- Capillary electrophoresis: 

                                         

 

 

 

 

 

 

 

*Rapid completely automated analysis

of up to 96 samples.

*Reduce time consumed.

*Accurate analysis of low concentration

nucleic acids with 3–5 bp resolution.

*It has been used for applications such as:

bacterial genotyping,

PCR fragment analysis, pre-sequencing testing.

 

 

8- Electrophoresis Unit that includes: 

                        

 

 

 

 

 

 

 

*Power supply. 

*Compact multi-wide horizontal gel

electrophoresis apparatus.

*Vertical electrophoresis system.

 

9- U.V gel transilluminator                                                

 

 

10- BioRad Gel Documentation System:

 

                   

 

 

 

 

 

 

 

*Easily visualization, documentation and

analysis of nucleic acid

and protein gels, blots and microarray.

*Adjustment of brightness, contrast and image filtering.

*Analysis of banding patterns and fingerprinting.

 

 

 

 

11- Water purification system:  

                                    

 

 

 

 

 

 

 

A direct system that  produces ultrapure (Type I)

water (16 L/h ; 320 L/ Day) for critical laboratory

indications as cell culture, HPLC,

immunocytochemistry and  in all steps of molecular

biology applications including DNA sequencing.

 

 

12- High Speed Cooling Centrifuge: (-8°C, 15,000 rpm)

      

 

 

 

13- Homogenizer: 

                                                            

 

 

 

 

 

 

 

MillMix 20 uses advanced technology

(DC brushless motor) for efficient disintegration,

homogenization and mixing of small amounts of

sample easily in seconds.

 

 

14- Bench top centrifuge:  

 

                                 

 

 

 

15- pH meter:

 

                                                            

 

 

 

 

16- Balance: highly sensitive 4 digits.

 

                                     

 

 

 

 

 

17-  Other Auxiliary Devices:                                               

    Vortex

•    Balance

•    Hot plat magnetic stirrer

 

 

 

18- Efficient cooling system

 

 

 

 

Previous Contributions and Research Projects

1.   Atheroscelorosis risk in patient with CKD :rol of cytotoxic T-lymphocyte and systemic inflammation to detect CD4,CD28 T cell by flowcytometry (with Nephrology Department in TBRI).

2.   Autologous bone marrow mesenchymal stem cell transplantation in patients with liver cirrohosis ( With Haematology Department  )

3.     In vitro expansion and differentiation of haematopoietic stem cells into platelets (with Haematology Department in TBRI).

4.     Autologous haematopoietic stem cell transplantation sub-terminal liver disease (with Haematology Department in TBRI).

 

 RunningResearch Projects

1- Detection of microRNA 223, 19a in HCC patient with HCV follow up and treatment of HCC patients(with Clinical Chemistry Department in TBRI).

2- Evolution of cytotoxicity(with Biochemistry Department in TBRI).

3-ABO blood group genotyping and risk of hepatocellular carcinoma in cohort of Egyptian patients (with Haematology Department in TBRI).

4- In vitro Activity of Antibiotic Combinations against Carbapenem Non-Susceptible Isolates of Acinetobacter baumannii and the Effects of their Antibiotic Resistance Genes (with Microbiology Department).

5-Detection of carbapenemase producing Enterobacteriaceae (CPE) in infected patients and colonized carriers (with Microbiology Department).

    Steps to reach facilities of central laboratory TBRI

1-    Notification of the central laboratory team member with the point of research.

2-    Specification of all equipment needed.

3-    Checking availability of chemicals required (The laboratory only offers basic chemicals). 

4-    Reservation of suitable working date.

5-    Filling request form application.