Overview


 The Schistosome Biological Supply Centre (SBSC) was established in 1989 at Theodor Bilharz Research Institute TBRI through a research collaborative agreement between the institute and Massachusetts -Lowell University, USA and was funded by the USAID.

Mission


Production of biological material required for scientific research as well as investigation of the efficiency of compounds with potential molluscicidal and schistosomicidal Activities.

Head and Staff Members


* Scientific Director: Emeritus Prof. Fatem Ramzy     

E mail: fatemg@hotmail.com

Tel. (202) 35406656 &35402549

Mobile:  01064629893

Founder and Former Head of the Center Late Emeritus Prof. Foad Youssef     

   *Professional Staff

Emeritus Prof. Fatem Ramzy,   Scientific and Technical Supervisor.

Prof. Samia El Bardicy ( PhD),

Emeritus Prof. Manal Kamel (MD) ,

Emeritus Prof. Kesmat Maher( MD),

Emeritus Prof. Soheir Mahmoud( PhD)

Emeritus Prof. Fathya Gawish

Assisst Prof. Menerva Maher

Assisst. Prof. Magda Atalla

Structure and Facilities


The center is formed of five laboratories: Snail Breeding Lab, Snail Infection Lab, Parasitology Lab, Antigen Production Lab, In Vitro Testing Lab, Guest Lab, Animal husbandry area and Information Unit. It is provided with the basic equipment such as, CO2 incubator, deep freezers, laminar flow, ultracentrifuge, lyophilizer, oven, double distillatory, electric balances, perfusion apparatus, autoclaves and stereomicroscopes, Ice maker, etc. The center is maintaining colonies of snails (Biomphalaria spp. and Bulinus truncatus), strains of mice (CD1, Balb C, C57BL 6) and golden hamsters imported from Charles River Lab. These animals are bred and maintained according to the universal ethics. Large cultures of Schistosoma mansoni and S. haematobium are also maintained.                                                                                 

 

 

 

 

 

 

  Snail Breeding Lab

 

  

Animal Husbandry

 

Field of Research


Producing the needed biological material for experimental and operational schistosomiasis research, including antigens of various stages Schistosoma mansoni and S. haematobium are also produced on large scale under standard conditions. This covers the needs of TBRI and these products are marketed to various institutions working on schistosomiasis, biology, biochemistry, immunology, operational research, etc.

  1. Testing various compounds of synthetic and natural (plants and marine organisms) origins on various stages of Schistosoma parasite and vector snails, aiming to discover new drugs and/or molluscicides.

Achievements


In vitro/In vivo evaluation of more than 11,000 compounds (active compounds and extracts) on various stages of Schistosoma parasite as well as on vector snails. This work was carried out in collaboration with WHO/ TDR, Egyptian Academy of Scientific Research, National Research Centre (Cairo) and Faculties of Pharmacy and Science in Egyptian Universities.

  1. Since 2003, SBSC is considered a WHO/TDR screening center for antiparasitic drugs and/or molluscicides.

 

 

 

 In vitro Screening Lab

Scope of Services


SBSC offers both services and consultation to various institutions in Egypt. 

  1. List of material produced and marketed by the center

ITEM

 

Unit

 

Snails

 

Biomphalaria alexandrina

 

Clean

100

 

Schisto-infected

10

 

Bulinus truncates

 

Clean

100

 

Schisto-infected

10

 

Lymnaea natalensis

 

Clean

100

 

Fasciola-infected

10

 

Schistosoma mansoni / S. haematobium

 

Worms

schistosomules

Viable or frozen, lyophilized

100 worms

 

Cercariae

Viable or fixed

1000

 

Eggs

Viable or fixed

10,000

 

Antigens

 

Worms

SWAP

1mg

 

Schistosomulae

SLAP

1mg

 

Cercariae

CAP

1mg

 

Eggs

SEA

!mg

 

Experimental animals

mice (CDI, C57BL6, Balb-c)  clean

5

 

infected (S.mansoni)

5

 

Golden hamsters   clean

1

 

Infected (S.mansoni) & (S.haematobium)

5

 

           

 

    *For requesting material,

     Please call Prof. Dr. Samia El Bardicy: Mobile: 01118621584

  1. List of various services available for scientists

ITEM

Estimation of Protein Content using BIO RAD assay kit.

Ultra filtration

Concentration of samples

Ultracentrifugation of samples up to 60000 Pm (1 hr)

Lyophilyzation ( 24 hrs)

Preparation of crude parasite antigens

Algae

Serum clean  mice

Serum clean  hamster

Serum immune S. mansoni (hamster)

Serum immune S.haematobium (hamster)

Serum immune S. mansoni (mice)

Mice Infection by various methods

Snail egg masses

O ogram

Ova Count/ gm tissue

Perfusion of animals  (Mouse or Hamster)

Picking of haematobium  adult  worms

Molluscicidal activity  &  LC determination

Cercaricidal activity

Miracidicidal activity

Schistosomicidal  activity In vitro &LC determination

Schistosomicidal  activity In vivo&LC determination

Schistosomules ( alive)

Training Facilities


Training workshops focus mainly on the preparation of biological material (Schistosoma & Fasciola), their utilization and maintenance in operational research as well as caring/breeding for experimental animals. Also, technical assistance on establishment of animal houses was offered to some Egyptian research institutions.                                                                                                                                                                               

  • The workshops were offered to trainees from Egypt, Middle East, Africa, Pakistan and USA. They were supported and funded by several international organizations including WHO, GTZ (Germany) and USAID.
  • The training workshops include lectures, practical techniques on the following topics:

a.       Breeding and maintenance of vector snails.

b.      Culture of algae for feeding snails.

  1. Exposure of snails to infection with miracidia under the utmost laboratory conditions.
  2. Maintenance and examination of infected snails.
  3. Cercarial production and study of intramolluscan larval stages.
  4. Infection of experimental animals with schistosome cercariae.
  5. Worm recovery methods from infected animals
  6. Egg separation from stools and tissues.
  7. Breeding and maintaining of mice and hamsters.

j.        Preparation of antigens of different stages of both parasites.

k.      Identification of snails and types of cercariae.

l.        Field visits to examine natural habitats and snails collection.