Vision


According to Egypt's Vision 2030, the medicinal chemistry laboratory has set a future research plan aimed to discovering Egyptian drugs and products from safe natural resources, either in the form of pharmaceutical raw materials or in the form of pure compounds to treat many diseases that fall within TBRI interest. Our vision also to make the laboratory a pioneer in the field of natural products chemistry and applied its research outputs  in cooperation with the industry which in turn will be benefit to society. Continuous research and development of methods for extraction and identification of pharmacologically leading compounds from natural resources. Transfer of experience and knowledge by providing a distinguished training service and collaborate researches, especially for researchers from Arab and African countries in the field of drug discovery from natural sources.

 

  Mission


Phytochemical and biological evaluation of natural products from different resources (plants, microbs, marines…etc) that could help in solve many problems facing society as treatment of diseases (drug discovery), industrial and ecological difficulties. Continuous learning and training for young researchers for raising their efficiency and developing their capabilities.

  Head of the Laboratory


 

 

Prof. El-Sayed Saleh Abdel-Hameed 
Email: shzssayed@yahoo.com

 

 

  Field of Research


a. Extraction of different natural plants classes: Saponin (steroidal and triterpenoidal), and phenolics (flavonoids, tannins, phenylethanoid, coumarins and volatile oils).


b. Isolation of different natural product compounds using different chromatographic techniques (HPLC, ordinary column chromatography, 2D- paper chromatography and TLC.


c. Detection of the nature of the isolated compounds by different spray reagents: for phenolic compounds (NH3, FeCl3 and Naturstoff), 10% sulphuric acid in methanol for saponin.


d. Structure elucidation of the pure compounds using chemical and spectroscopic analysis as IR, different types of mass spectrometry (EI, FAB, CI-ESI and HRESI-Ms), 1 and 2D NMR experiments like (e.g. 1H-1H, COSY, HMBC, HMQC).


e. Biological application of antischistosomal, molluscicidal antimicrobial, antioxidant, hepatoprotective, anti-inflammatory, cytotoxic and nephrotoxicity activates of the collected plants under experiments as a dry powder and/or extracts and as a pure isolated compounds. 

  Main Achievements


a. Evaluation of anticancer and antiviral activity of methanol extracts and their derived chloroform and n-butanol extracts from both plants.

b. Evaluation and chromatographic isolation of over than 400 plants of different biological applications.

c.  Contribution to GenBank:

·         Aspergillus fumigatus 3T-EGY/ Accession no. KP140961
http://www.ncbi.nlm.nih.gov/nuccore/KP140961

·         Geomyces pannorum ATS2-EGY/ Accession no. KP109702
http://www.ncbi.nlm.nih.gov/nuccore/KP109702

·         Penicillium sp. SAM16-EGY/ Accession no. KP125952
http://www.ncbi.nlm.nih.gov/nuccore/KP125952

·         Eurotium rubrum 2TS-EGY/ Accession no. KP120893
http://www.ncbi.nlm.nih.gov/nuccore/KP120893

  Running Techniques


a. Chromatographic isolation [HPLC-DAD-ESI-MS/MS, Glass column, Paper Chromatography, TLC] on silica gel, polyamide, Sephadex, cellulose, etc...
b. Different antioxidant techniques.
c. Assessment of cytotoxic activity via brine shrimp method.
d. Molluscicidal activity.
e. Hepatoprotective activity.  

  Laboratory Structure and Facilities


 The department has the following equipment: Rotatory evaporator, balances, High Performance Liquid Chromatography (HPLC), Glass column chromatography, Ultraviolet Lamp, UV-visible spectrophotometer, Melting Point apparatus, Centrifuge, Electric oven, Vacuum pump, pH meter, water bath, Electric Grinder, heater and shaker.

  Training facilities


 a. Importance and evaluation of medicinal plants and natural products.

b. Chromatographic isolation of natural products constituents [HPLC, Glass column, Paper Chromatography, Thin layer chromatography] on silica gel, polyamide, Sephadex, cellulose…..ect.

c. Spectroscopic analysis of the isolated natural product compounds.

d. Different antioxidant techniques.

e. Measuring cytotoxicity using brine shrimp method.

f. Molluscicidal activity.

  Projects


* Internal Projects

a. Pilot Study of Anticancer Potential of Some Herbal Medicine. PI:  Prof. Hanaa Ismail, Immunology Department (2008).

b. Bioassay of Methanol Extracts from Certain Egyptian Plant species against Biomphalaria alexandrina snails and their infection with Schistosoma mansoni. PI: Prof. Hoda Abdel-Hamid, Medical Malacology Department (2012)

c. Bioactivity of certain secondary metabolites from some Egyptian plants and fungi species. PI: Prof. Laila Refahy (2013).

d. Chemical and biological investigation of Gmelina arborea and Tectona grandis (family Verbenaceae). PI: Prof. Laila Refahy (2013).

e. Chromatographic isolation and identification of the poly phenolic and triterpenoidal saponins compounds from two Cestrum species growing in Egypt. PI: Prof. Mona Abdel-Motagally Mohamed (2016).

f. The Role of Some Plants extracts in Raising Resistance of Snails to Infection with Trematodes ''. PI: Assoc. Prof. Hanan Shahat Mossalem, Medical Malacology Laboratory (2015).

g.  Chromatographic separation of certain chemical constituents of two Salix species and evaluating them as antioxidant and antimicrobial agents.  PI: Prof. Mortada El-Sayed (2015).

h.  Isolation and Identification of some chemical constituents of Cinnamomum zeylanicum and Psidium guajava and evaluation of their biological activity. PI: Prof.  Samir Mohamed El-Amin (2016).

i.   Phytochemical studies on Cucurbita pepo and Lantana camara extract and evaluating them as nephroprotective agent using rat model. PI: Prof. Mortada El-Sayed (2015).

* External Projects

a.  SIDA, Lund University, Sweden (2009-2011):  Developments of new compounds with effect on Schistosomiasis (Bilharzias). PI: Prof. Sanaa Botros,  Pharmacology Department  

b. Science and Technology Development Fund (STDF) (2012-2014): Extraction and chromatographic isolation of certain chemical constituents of two Allium species and their evaluation as antioxidant and cytotoxic agents. PI: Dr. Ezzat El-Sayed.

c.  STDF (2014-2016): Recent approaches in the utilization of Moringa oleifera and Moringa peregrina as a good nutritional, medicinal and industrial plant in Egypt. PI: Prof. Aboul Fotouh Mohamed Abdullah, Horticultural Technology Department-Division of Agricultural Sciences, National   Research Center.

d. MHESR (2015-2017): Isolation and screening of actinobacteria from Moroccan-Egyptian ecosystems as biological control agents against the crown and root rot of tomato caused by Fusarium oxysporum f.sp. (radices-lycopersici) Sacc. PI: Prof. Mohamed El-Sayed Abdel-Aziz, Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National   Research Center.

e. NRC (2015-2018): Application of biotechnology to reduce mycotoxin problems. PI: Prof. Aml Shawky Hathot, Department of Toxins and Pollutants Food, Food Industry and Nutrition Division, National   Research Center.

f. NRC (2016-2019): Use of Bacterial Lipase Enzyme in Bio-Fuel Matrix Production from Microalgae. PI: Prof. Abo El-Khair B. El-Sayed, Department of Fertilization Technology, Agricultural and Biological Division, National Research Center.

g. NRC (2016-2019):  Bioactive materials from agriculture wastes for upgrading the functional properties of cellulose containing textiles. PI: Prof. Basma M. Eid, Department: Preparation and Finishing of Cellulosic Fibers, Textile Research Division, National Research Center.

h. NRC (2016-2019):   Elimination of the toxicity of nanoparticles used in food sector. PI: Prof. Mosaad Attia Abdel-Wahhab, Department: Food Toxicology & Contaminants, Food Industry & Nutrition Division, National Research Center.

i. STDF and Egypt UK Newton Musharafa Fund (2017-2019): Antimicrobial secondary metabolites from marine endophytic fungi for functionalization of textiles. PI: Dr. Ahmed Abdelghani Abdalla, Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National   Research Center. 

  Publications


  1. Eman Abdullah Morsi, Hend Okasha, Heba Abdel Hady, Mortada El-Sayed, Mohamed Abbas Shemis (2020): Gas Chromatography-Analysis, Antioxidant, Anti-Inflammatory, and Anticancer Activities of Some Extracts and Fractions of Linum usitatissimum. Current Bioactive Compounds (accepted and under press).

  2. Ghareeb MA, Khalaf OM, Abdel-Aziz MS, Saad AM, Madkour HMF, El-Ziaty AK, Refahy LA. (2020): Chemical profiles and bio-activities of different extracts of Terfezia species and their other associated fungi. Current Bioactive Compounds, 16(3): 308-319.

  3. Cheraif K, Bakchiche B, Gherib A, Bardaweel SK, Ayvaz MC, Flamini G, Ascrizzi R,  Ghareeb MA. (2020): Chemical composition, antioxidant, anti-tyrosinase, anti-cholinesterase and cytotoxic activities of essential oils of six Algerian plants. Molecules, 25(7): 1710.

  4. Ibrahim AM, Ghareeb MA.(2020):. Preliminary phytochemical screening, total phenolic content, in vitro antioxidant and molluscicidal activities of the methanolic extract of five medicinal plants on Biomphalaria alexandrina snails. Journal of Herbs, Spices & Medicinal Plants, 26(1): 40-48.

  5. Wei Jiang, Juan Xiong, Yi Zang, Junmin Li, Ezzat E. A. Osman, Jing-Ya Li, Yu-Bo Zhou, Jia Li, Jin-Feng Hu. (2020): Phytochemical and biological studies on rare and endangered plants endemic to China. Part XIV. Structurally diverse terpenoids from the twigs and needles of the endangered plant Picea brachytyla. Phytochemistry, 169: 112161.

  6. Shaltout AA, Abdel-Hameed ES, Bilo F.,  Borgese  L., Depero L. (2020): Direct analysis of essential oils by means of TXRF spectrometry. X-Ray Spectrometry, 49: 322–331.

  7. Marzouk, M.M., Ibrahim, L.F., El-Hagrassi, A.M., Fayed D.R.,  Elkhateeb A, Abdel-Hameed E.S., Hussein SR., (2020): Phenolic profiling and anti-Alzheimer’s evaluation of Eremobium aegyptiacum. Advances in Traditional Medicine 20:233–241.

  8. Yu Tang, Na Li, Yike Zou, Yanran Ai, Guang-Lei Ma, Ezzat E. A. Osman, Juan Xiong, Junmin Li, Ze-Xin Jin, Jin-Feng Hu.(2019): LC-MS guided isolation and dereplication of Lycopodium alkaloids from Lycopodium cernuum var. sikkimense of different geographical origins. Phytochemistry, 160: 25-30.

  9. Wafaa S. Ahmed, Ezzat E. Abdel-Lateef, Eman A. El-Wakil, EL-Sayed S. Abdel-Hameed (2019): In vitro antioxidant and antimicrobial properties of Murraya paniculata L. extracts as well as identification of their active secondary metabolites by HPLC-ESI-MS. Der Pharma Chemica, 11(3): 1-8.

  10. Mohammed HS, Abdel-Aziz MM, Abu-baker MS, Saad AM, Mohamed MA, Ghareeb MA. (2019): Antibacterial and potential antidiabetic activities of flavone C-glycosides isolated from Beta vulgaris subspecies cicla L. var. flavescens (Amaranthaceae) cultivated in Egypt. Current Pharmaceutical Biotechnology, 20(7): 595-604.

  11. Marzouk MM., Elkhateeb A, Abdel Latif RR., Abdel-Hameed E.S., Mona El-Shabrawy M, Abdel-Rahman R.F., El-Desoky A.H., Hussein SR., Kawashty SK. (2019): C-glycosyl flavonoids-rich extract of Dipcadi erythraeum Webb & Berthel. bulbs: Phytochemical and anticancer evaluations. Journal of Applied Pharmaceutical Science Vol. 9(06): 094-098.

  12. Manal M. Hamed, Mosad A. Ghareeb, Azza A. Shafei, Mohamed S. Abdel-Aziz, Samar S. Tolba (2019). The in vitro evaluation of antioxidant, anticancer and antimicrobial properties of Araucaria heterophylla grown in Egypt. Pharmacology online 1: 221-235.

  13. Manal M. Hamed, Mohammed A. Abd El-Mobdy, Marian T. Kamel, Heba I. Mohamed, Abdelrahman E. Bayoumi (2019). Phytochemical and Biological Activities of Two Asteraceae Plants Senecio vulgaris and Pluchea dioscoridis L. Pharmacology online 2: 101-121.

  14. Khalaf OM, Ghareeb MA, Saad AM, Madkour HMF, El-Ziaty AK, Abdel-Aziz MS. (2019): Phenolic constituents, antimicrobial, antioxidant and anticancer activities of ethyl acetate and n-butanol extracts of Senna italica. Acta Chromatographica, 31(2): 138-145.

  15. Juan Xiong, Wei-Jia Meng, Hai-Yan Zhang, Yike Zou, Wen-Xuan Wang, Xin-Yi Wang, Qi-Lian Yang, Ezzat E. A. Osman, Jin-Feng Hu. (2019): Lycofargesiines A–F, further Lycopodium alkaloids from the club moss Huperzia fargesii. Phytochemistry, 162: 183-192.

  16. Heba Abdel-Hady, Eman Ahmed El-Wakil, Sami Mohamed Nasr (2019):  Characterization and Evaluation of Antimicrobial and Cytotoxic Activities of Glycine max Methanol Extract. Inter. J. Pharm. Res. 11(2):418-426,

  17. Heba Abdel-Hady, Eman Ahmed El-Wakil, Eman Abdalla Morsi (2019):Characterization of ethyl acetate and methanol extracts of Commiphora  myrrha and evaluating in-vitro anti-diabetic and anti-obesity activities. J. Appl. Pharma. Sci., 9(9):38-44.

  18. Ghareeb MA, Hamed MM, Saad AM, Abdel-Aziz MS, Hamed AA, Refahy LA. (2019): Bioactive secondary metabolites from the locally isolated terrestrial fungus. Penicillium sp. SAM16-EGY. Pharmacognosy Research, 11(2): 162-170.

  19. Eman Ahmed El-Wakil, Eman Abdalla Morsi, Heba Abdel-Hady (2019): Phytochemical screening, antimicrobial evaluation and GC-MS analysis of Cyperus rotundus.. World J. Pharm. Pharm. Sci., 8(9): 129-139.

  20. Shawky BT, Nagah M, Ghareeb MA, El-Sherbiny GM, Moghannem SAM, Abdel-Aziz MS. (2019): Evaluation of antioxidants, total phenolics and antimicrobial activities of ethyl acetate extracts from Fungi grown on rice straw. Journal of Renewable Materials, 7(7): 667-682.

  21. Safyah B. Bakare, Mosad A. Ghareeb. (2019):  Chemical profile, antimicrobial and antioxidant activities of Moringa oleifera Lam leaves grown in Saudi Arabia. Mens Agitat, 14: 45-52.

  22. Ghareeb MA, Sobeh M, El-Maadawy WH, Mohammed HS, Khalil H, Botros SS, Wink M. (2019): Chemical profiling of polyphenolics in Eucalyptus globulus and evaluation of its hepato-renal protective potential against cyclophosphamide induced toxicity in mice. Antioxidants, 8(9): 415.

  23. Elkhateeb A, Mona El-Shabrawy M, Abdel-Rahman R.F.,  Marzouk MM., El-Desoky A.H., Abdel-Hameed E.S., Hussein SR. (2019): LC-MS-based metabolomic profiling of Lepidium coronopus water extract, anti-inflammatory and analgesic activities, and chemosystematic significance. Medicinal Chemistry Research, 28: 505-514.

  24. Bakchiche B, Gherib A, Bronze MR, Mosad A. Ghareeb MA. (2019): Identification, quantification, and antioxidant activity of hydroalcoholic extract of Artemisia campestris from Algeria. Turkish Journal of Pharmaceutical Sciences, 16(2): 234-239.

  25. Abdel-Wareth MTA, Ghareeb MA, Abdel-Aziz MS, El-Hagrassi AM. (2019): Snailicidal, antimicrobial, antioxidant and anticancer activities of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus fungal extracts.  Egyptian Journal of Aquatic Biology & Fisheries Zoology, 23(2): 195 -212.

  26. Abdel-Wareth MTA, El-Hagrassi AM, Abdel-Aziz MS, Nasr SM, Ghareeb MA. (2019): Biological activities of endozoic fungi isolated from Biomphalaria alexandrina snails maintained in different environmental conditions. International Journal of Environmental Studies, 76(5): 780-799.

  27. Sobeh M, Mahmoud MF, Hasan RA, Abdelfattah MAO, Sabry OM, Ghareeb MA, El-Shazly AM, Wink M. (2018): Tannin-rich extracts from Lannea stuhlmannii and Lannea humilis (Anacardiaceae) exhibit hepatoprotective activities in vivo via enhancement of the anti-apoptotic protein Bcl-2. Scientific Reports, 8: 9343.

  28. Mossalem HS, Habib MR and Ghareeb MA (2018). Control of infection of Biomphalaria alexandrina (Ehrenberg, 1831) with Schistosoma mansoni (Sambon, 1907) using Eucalyptus camaldulensis. Folia Malacologica, 26(3): 1-11.

  29. Ghareeb MA, Sobeh M, Rezq S, El-Shazly AM, Mahmoud MF  and Wink M (2018). HPLC-ESI-MS/MS profiling of polyphenolics of a leaf extract from Alpinia zerumbet (Zingiberaceae) and its anti-Inflammatory, anti-nociceptive, and antipyretic activities in vivo. Molecules, 23: 3238.

  30. Abdel-Wareth MTA and Ghareeb MA (2018). Bioprospecting certain freshwater-derived fungi for phenolic compounds with special emphasis on antimicrobial and larvicidal activity of methyl gallate and p-coumaric acid. Egyptian Journal of Chemistry, 61(5): 500-510.

  31. Shaltout AA, Abdel-Hameed ES, Salman MS, Kregsamer P, Wobrauschek P, Streli C (2018).  Method Development and Quantitative Elemental Analysis of Mentha Longifolia L. Leaves from Saudi Arabia by Total Reflection X-Ray Fluorescence. Analytical Letters, 51(9): 1433-1444.

  32. Sami M. Nasr, Mosad A. Ghareeb,  Mona A. Mohamed , Nehal M. Elwan, Abd El-Wanes Anter Abdel-Aziz, Mohamed S. Abdel-Aziz (2018) High-Performance Liquid Chromatography-Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Two Cestrum Species Growing in Egypt, Pharmacognosy Research,10: 173-180.

  33. Manal Mortady Hamed (2018). Antidiabetic Activity of Medicinal Plants. International Journal of Pharmaceutical Sciences Review and Research, 51(1): 151-165.

  34. Salwa Fouad Oshiba , Rania Ibrahim Yassien, Amal Mohamed El- Shennawy, Hayam Abdel Samie Aiad and Eman Ahmed El-Wakil  (2018). Vivo effect of pomegranate (Punica granatum) extracts versus nitazoxanide drug on the ileum of experimentally infected mice with Cryptosporidium parvum oocysts.  Journal of American Science, 14(2): 27-39.

  35. Salman MS, Abdel-Hameed ES, Alshehri A (2018). A GC/MS Comparison Study of Pelargonium graveolens Essential Oils Extracted by Hydrodistillation and Microwave Assisted Hydrodistillation from North Regions in Kingdom of Saudi Arabia. Der Pharma Chemica, 10(6): 28-35.

  36. Safwat Gehan Mohamed, Manal Mortady Hamed, Helmy Aseel Talaat (2018). The biological activity of Conocarpus erectus extracts and their applications as cytotoxic agents. Pharmacology online 2: 171-184.

  37. Safwat Gehan Mohamed, Hamed Manal Mortady, Moatamed Shrouk Atef (2018). Studies of the biological activity of Cassia fistula. Pharmacology online, 1: 75-85.

  38. Marzouk MM, Hussein SR, Elkhateeb A, El-Shabrawy M, Abdel-Hameed ES and Kawashty SA (2018).  Comparative study of Mentha species growing wild in Egypt: LC-ESI-MS analysis and chemosystematic significance. Journal of Applied Pharmaceutical Science, 8(08): 116-122.

  39. Hussein SR, Abdel Latif RR.,  Marzouk MM, Elkhateeb A,  Mohammed RS,  Soliman AA, Abdel-Hameed ES (2018). Spectrometric analysis, phenolics isolation and cytotoxic activity of Stipagrostis plumosa (Family Poaceae). Chemical Paper, 72 (1): 29–37.

  40. Heba Abdel-Hady, Mortada M. El-Sayed, Mahfouz M. Abdel-Gawad, Eman A. El-Wakil, EL-Sayed S. Abdel-Hameed, Ezzat E. Abdel-Lateef (2018): LC-ESI-MS analysis, antitumor and antioxidant activities of methanolic extract of Egyptian Allium kurrat. J. Appl. Pharm. Sci., 8 (7): 85-92.

  41. Heba Abdel-Hady, Eman Ahmed El-Wakil and Mahfouz Abdel-Gawad (2018). GC-MS Analysis, Antioxidant and Cytotoxic Activities of Mentha spicata. European Journal of Medicinal Plants. 26(1): 1-12

  42. Mortada M. El-Sayed, Nadia H. Metwally, Ibrahim A. Ibrahim, Heba Abdel-Hady, Bassant S.A. Abdel-Wahab (2018). Antioxidant activity, total phenolic and flavonoid contents of Petroselinum crispum Mill. J. Appl. Life. Sci. Int. 19 (2): 1-7.

  43. Hamed M M, Refahy L A, Shafei A A, Abd El Aziz E G I.(2018):  Antimicrobial Activity and Chemical Composition of Essential Oil from Pittosporum eugenioides. Int. J. Pharm. Sci. Rev. Res., 51(1): 187-192.

  44. Guang-Lei Ma, Juan Xiong, Ezzat E. A. Osman, Ting Huang, Guo-Xun Yang, Jin-Feng Hu. (2018): LC-MS guided isolation of sinodamines A and B: Chimonanthine-type alkaloids from the endangered ornamental plant Sinocalycanthus chinensis. Phytochemistry, 151: 61-68.

  45. Ghareeb MA, Saad AM, Ahmed WS, Refahy LA, Nasr SM. (2018): HPLC-DAD-ESI-MS/MS characterization of bioactive secondary metabolites from Strelitzia nicolai leaf extracts and their antioxidant and anticancer activities in vitro. Pharmacognosy Research, 10(4): 368-378.

  46. Ghareeb MA, Mohamed T, Saad AM, Refahy LA, Sobeh M, Wink M. (2018): HPLC-DAD-ESI-MS/MS analysis of fruits from Firmiana simplex (L.) and evaluation of their antioxidant and antigenotoxic properties. Journal of Pharmacy and Pharmacology, 70: 133-142.

  47. Farid MM, Marzouk MM, Hussein SR, Elkhateeb A and Abdel-Hameed ES (2018). Comparative study of Posidonia oceanica L.: LC/ESI/MS analysis, cytotoxic activity and chemosystematic significance. Journal of Materials and Environmental Sciences, 9(6), 1676-1682.

  48. Ezzat E. Abdel-Lateef, Ibrahim A. Rabia, Mahfouz M. Abdel-Gawad, Mortada M. El-Sayed (2018). In vitro antischistosomal activity of Allium cepa L. (Red onion) extracts and identification of the essential oil chemical composition by GC-MS. J. Microbiol. Biotech. Food Sci. 7 (4): 421-425.

  49. El-Sayed, M. M., Abdel-Aziz, M. M.,  Abo-Sedra, S. A., Mohamed, H. R., & Abdel-lateef, E. E. (2018). Isolation and identification of certain phenolic compounds from Salix mucronata leaf extracts and evaluation them as antimicrobial agents. Current Bioactive Compounds, 15(3), 360–366.

  50. Aladwani, AM. Salman M and Abdel Hameed ES. (2018): Chemical composition of Ocimum basilicum L. essential oil from different regions in the Kingdom of Saudi Arabia by using Gas chromatography mass spectrometer. Journal of Medicinal Plants Studies, 6(1): 14-19.

  51. Abdel-Lateef EE,  Rabia IA, El-Sayed MM and Abdel-Hameed ES (2018). HPLC-ESI-MS Characterization of Certain Polyphenolic Compounds of Carica papaya L. Fruit Extracts and Evaluation of Their Potential Against Murine Schistosomiasis mansoni. Drug Research, 68(9):521-528.

  52. Abdel-Hameed ES, Salman MS, Fadl M.A, Elkhateeb A, and Hassan MM (2018). Chemical composition and biological activity of Mentha longifolia L. solvent free Microwave and microwave hydrodistillation. Journal of Essential oil Bearing Plants, 21(1): 1-14.

  53. Abdel-Hameed ES, Salman MS, Fadl M.A, Elkhateeb A, and El-Awady MA (2018). Chemical Composition of Hydrodistillation and Solvent Free Microwave Extraction of Essential Oils from Mentha piperita L. Growing in Taif, Kingdom of Saudi Arabia, and their Anticancer and Antimicrobial Activity. Oriental Journal of Chemistry, 34(1):  222-233.

  54. Abdel-Hady H, El-Sayed M, Abdel-hady AA, Hashash MM, Abdel-Hady AM, Aboushousha T, Abdel-Hameed ES, Abdel-Lateef EE, Morsi EA. (2018). Nephroprotective Activity of Methanolic Extract of Lantana camara and Squash (Cucurbita pepo) on Cisplatin-Induced Nephro­toxicity in Rats and Identification of Certain Chemical Constituents of Lantana camara by HPLC-ESI-MS. Pharmacognosy Journal, 10(1):136-147.

  55. Abdel-Aziz MS, Ghareeb MA, Saad AM, Refahy LA, Hamed AA. (2018): Chromatographic isolation and structural elucidation of secondary metabolites from the soil-inhabiting fungus Aspergillus fumigatus 3T-EGY. Acta Chromatographica. 30(4): 243-249.

  56. Saad, A.M., Mohammed, M.M.D., Ghareeb, M.A., Ahmed, W.S., Farid, M.A. (2017): Chemical composition and antimicrobial activity of the essential oil of the leaves of Cupressus macrocarpa Hartweg. ex Gordon. Journal of Applied Pharmaceutical Science, 7, 207-212.

  57. Hamed, M.M., Ghareeb, M.A., Abdel-Aleem, A.H., Saad, A.M., Abdel-Aziz, M.S., Hadad, A.H. (2017): Chemical analysis, antioxidant activity and antimicrobial activity of isolated compounds and essential oil from Callistemon citrinus leaf,“ International Journal of Pharmaceutical Sciences Review and Research, 44, 209-216.

  58. Hamed, M.M., Yossef, A.M.A., Ghareeb, M.A., Saleh, S.A. (2017): Chemical constituents, in vitro antioxidant activity, oral acute toxicity and LD50 determination of Moringa oleifera leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 9, 240-247.

  59. Saad, A.M., Ghareeb, M.A., Abdel-Aziz, M.S., Madkour, H.M.F., Khalaf, O.M., El-Ziaty, A.K., Abdel-Mogib, M. (2017): Chemical constituents and biological activities of different solvent extracts of Prosopis farcta growing in Egypt.  Journal of Pharmacognosy and Phytotherapy, 9, 67-76.

  60. Madkour, H.M.F., Ghareeb, M.A., Abdel-Aziz, M.S., Khalaf, O.M., Saad, A.M., El-Ziaty, A.K., Abdel-Mogib, M. (2017): Gas chromatography-mass spectrometry analysis, antimicrobial, anticancer and antioxidant activities of n-hexane and methylene chloride extracts from Senna italic. Journal of Applied Pharmaceutical Science, 7, 023-032.

  61. Hamed, M.M., Refahy, L.A, Abdel-Aziz, M.S. (2017): Assessing the bioactivity and antioxidative properties of some compounds isolated from Abutilon hirtum (Lam.). Asian Journal of Pharmaceutical and Clinical Research. 10 (3): 333 – 340.

  62. Ghareeb, M.A., Hamed, M.M., Abdel-Aleem, A.H., Saad, A.M., Abdel-Aziz, M.S., Hadad, A.H. (2017): Extraction, isolation and characterization of bioactive compounds and essential oil from Syzygium jambos. Asian Journal of Pharmaceutical and Clinical Research, 10, 194-200.

  63. Ismail, O.M.M., Abdel-Aziz, M.S., Ghareeb, M.A., Hassan, R.Y.A. (2017): Exploring the biological activities of the Hylocereus polyrhizus extract. Journal of Innovations in Pharmaceutical and Biological Sciences, 4, 01-06.

  64. Mossalem, H.S., Ghareeb, M.A., Refahy, L.A., Mohamed, A.S., Habib, M.R. (2017): Gas chromatography-mass spectrometry analysis and antioxidant activity of Punica granatum L. peels and its role as immunostimulant against Schistosoma mansoni infection in Biomphalaria alexandrina,“ Asian Journal of Pharmaceutical and Clinical Research, 10, 1-7.

  65. Heba Abdel-Hady, Mahfouz Mohamed Abdel-Gawad, Eman Ahmed El-Wakil (2017). Characterization and evaluation of antioxidant activity of Ocimum canun leaves and its efficiency on Schistosoma mansoni larval stage. INDO American J of Pharmaceutical Reseach. Vol. 7 (11).

  66. Mortada M. El-Sayed, Maher M. Hashash, Afaf A. Abdel-Hady, Heba Abdel-Hady, Ezzat E. Abdel-Lateef and Eman A. Morsi (2017). Total phenolic and flavonoid contents and antioxidant activity of Lantana camara and Cucurbita pepo (squash) extracts as well as GC-MS analysis of Lantana camara essential oils. World J. Pharm. Res. 6(1), 137-153.

  67. Saad, A.M., Abdel-Aleem, A.H., Ghareeb, M.A., Hamed, M.M., Abdel-Aziz, M.S., Hadad, A.H. (2017): In vitro antioxidant, antimicrobial and cytotoxic activities and green biosynthesis of silver & gold nanoparticles using Callistemon citrinus leaf extract. Journal of Applied Pharmaceutical Science, 7, 141-149.

  68. El-Hagrassi AM, Elkhateeb A, Hussein SR, Abdel-Hameed ES, Marzouk MM. (2017):  LC–ESI-MS profile, antioxidant activity and cytotoxic screening of Oligomeris linifolia (Vahl) Macbr. (Resedaceae). J App Pharm Sci, 7 (08): 043-047.

  69. El-Sayed S. Abdel-Hameed, Salih A. Bazaid (2017): Chemical composition of essential oil from leaves of Schinus molle L. growing in Taif, KSA. Journal of Essential Oil Bearing Plants, 20 (1); 45 – 58.

  70. Maher M. Hashash, Mortada M. El-Sayed, Afaf A. Abdel-Hady, Heba Abdel Hady, Eman A. Morsi (2017). Nutritional potential, mineral composition and antioxidant activity squash (Cucurbita pepo L.) fruits grown in Egypt.  Europ.  J. Biomed. Pharma. Sci. 4 (3), 05-12.

  71. Hamed, M., M. Mohamed, and W. Ahmed. (2017): Antioxidant Potential, Antitumor Activity and Phenolic Profile of Phoenix Dactylifera Linn”. International Journal of Pharmacy and Pharmaceutical Sciences, 9(5): 130-136.

  72. Mohamed A. El-Awady, Mohamed M. Hassan, El-Sayed S. Abdel-Hameed and Ahmed Gaber (2016): Comparison of the antioxidant activities, phenolic and flavonoids contents of the leaves-crud extracts of Moringa peregrine and Moringa oleifera. International Journal of Biosciences, 8 (1) 55-62.

  73. Lamyaa F. Ibrahim, Ahmed Elkhateeb, Mona M. Marzouk, Sameh R. Hussein,  El-Sayed S. Abdel-Hameed and Mona E. S. Kassem (2016): Flavonoid investigation, LC–ESI-MS profile and cytotoxic activity of Raphanus raphanistrum L. (Brassicaceae). J. Chem. Pharm. Res., 8(7):786-793.

  74. Mona M. Marzouk, Sameh R. Hussein, Ahmed Elkhateeb, Mai M. Farid, Lamyaa F. Ibrahim and El-Sayed S. Abdel-Hameed (2016): Phenolic profiling of Rorippa palustris (L.) Besser (Brassicaceae) by LC-ESI-MS: Chemosystematic significance and cytotoxic activity. Asian Pac. J. Trop. Dis., 6(8): 633-637.

  75. Heba Abdel-Hady, Marwa Tamim Ahmed Abdel-Wareth, Eman Ahmed El-Wakil, Eman Ahmed Helmy (2016). Identification and evaluation of antimicrobial and cytotoxic activities of Penicillium islandicum and Aspergillus tamarii ethyl acetate extracts. World Journal of Pharmacy and Pharmaceutical Sciences. Vol. 5 (09), pp. 2021-2039.

  76. Ghareeb, M.A., Refahy, L.A., Saad. A.M., Ahmed, W.S., (2016): Chemical composition, antioxidant and anticancer activities of the essential oil from Eucalyptus citriodora (Hook.) leaves. Der Pharma Chemica,  8, 192-200.

  77. El-Neekety, A.A., Abdel-Aziz, M.S., Hathout, A.S., Hamed, A.A., Sabry, B.A., Ghareeb, M.A., Aly, S.E., Abdel-Wahhab, M.A.,  (2016): Molecular identification of newly isolated non-toxigenic fungal strains having antiaflatoxigenic, antimicrobial and antioxidant activities. Der Pharma Chemica, 8, 121-134.

  78. Hathout, A.S., EL-Neekety, AA, Abdel Aziz, M.S., Sabry, B.A., Hamed, A.A., Ghareeb, M.A., Aly, S.E. (2016): Novel Egyptian bacterial exhibiting antimicrobial and antiaflatoxigenic activity,“ Journal of Applied Pharmaceutical Science, 6, 001-010.

  79. Manal M Hamed, Mona A Mohamed, Magda T Ibrahim (2016): Cytotoxic Activity Assessment of Secondary Metabolites from Tecomaria capensis v. aurea. International Journal of Pharmacognosy and Phytochemical Research 8(7); 1173-1182.

  80. Samir M. El-Amine, Maher A.M. Hashash, Allia M. Abdou, Amal M.Saad, Mohamed S.Abdel-Aziz and Asmaa S. Mohamd (2016): Antimicrobial and antioxidant activities of Psidium Guajava leaves growing in Egypt. Der Pharmacia Letter, 8(12):27-33.

  81. Ghareeb, M.A., Ahmed, W.S., Refahy, L.A., Abdou, A.M., Hamed, M.M., Abdel-Aziz, M.S. (2016): Isolation and characterization of the bioactive phenolic compounds from Morus alba L. growing in Egypt,Pharmacologyonline, 3, 157-167.

  82. Abd El-Rahman A.A. A., I.M. Abd El-Aleem, L.A. Refahy and M.A. El-Shazly. (2016):  Total phenolic content, cytotoxic and antioxidant activities of Quisqualis indica (Linn.) growing in Egypt. Der Pharma Chemica, 8(3):53-59.

  83. Ezzat E. Abdel-Lateef, Olfat A. Hammam, Faten S. Mahmoud, Shima A. Atta, Mortada M. El-Sayed, Hanaa I. Hassenein (2016). Induction of apoptosis in human hepatoma cell lines (HepG2) by Vitex agnus-castus L. leaves extracts and identification of their active chemical constituents by LC-ESI-MS. Asian Pac. J. Trop. Dis. 6(7): 539- 548.

  84. Ezzat E. Abdel-Lateef, Faten S. Mahmoud, Olfat A. Hammam, Eman G. El-Ahwany, Eman A. El-Wakil, Sherihan Kandil, Hoda Abu Taleb, Mortada M. El-Sayed, Hanaa I. Hassenein (2016). Bioactive chemical constituents of Curcuma longa L. extract inhibit the growth in human hepatoma cell line (HepG2). Acta Pharm. 66: 387-398.

  85. Ghareeb, M.A., Saad, A.M., Abdou, A.M., Refahy, L.A., Ahmed, W.S. (2016): A new kaempferol glycoside with antioxidant activity from Chenopodium ambrosioides growing in Egypt. Oriental Journal of Chemistry, 32, 3053-3061.

  86. Ghareeb, M.A., Saad, A.M., Abdel-Aleem, A.H., Abdel-Aziz, M.S., Hamed, M.M., Hadad, A.H. (2016): Antioxidant, antimicrobial, cytotoxic activities and biosynthesis of silver & gold nanoparticles using Syzygium jambos leaves growing in Egypt. Der Pharma Chemica, 8, 277-286,

  87. El-Sayed S. Abdel-Hameed, Salih A. Bazaid and Heba A. Hagag (2016): Chemical characterization of Rosa damascena Miller var. trigintipetala Dieck essential oil and its in vitro genotoxic and cytotoxic properties. Journal of Essential Oil Research, 28( 2), 121–129.

  88. Mohamed Shohayeb, Hassan Arida, El-Sayed S. Abdel-Hameed and Saleh Bazaid (2015): Effects of Macro- and Microelements in Soil of Rose Farms in Taif on Essential Oil Production by Rosa damascena Mill. Journal of Chemistry, Volume 2015, Article ID 935235, 7 pages.

  89. Mohamed Abdelhamid El-Awady, Mohamed Mahmoud Hassan, El-Sayed Saleh Abdel-Hameed, and Ahmed Gaber (2015): Comparison of the Antimicrobial Activities of the Leaves-Crude Extracts of Moringa peregrina and Moringa oleifera in Saudi Arabia. Int. J. Curr. Microbiol. App. Sci., 4(12): 1-9.

  90. Mohamed A. Fadle; El-Sayed S. Abdel-Hameed; Hussein F. Farrag; Salih A. Bazaid (2015): Total phenolics and antioxidant activity of extracts from the leaf samples of wild African and cultivated Mediterranean olive growing in Taif, KSA and their taxonomical significance. Der Pharmacia Lettre, 7 (5):93-103.

  91. Mahmood Salman;  El-Sayed S. Abdel-Hameed;  Salih A. Bazaid; and Marwah M. Dabi (2015): Chemical composition for hydrodistillation essential oil of Mentha longifolia by gas chromatography-mass spectrometry from north regions in Kingdom of Saudi Arabia. Der Pharma Chemica, 7(4):34-40.

  92. Ghareeb, M.A., Refahy, L.A., Saad, A.M., Osman, N.S., Abdel-Aziz. M.S., El-Shazly, M.A., Mohamed, A.S. (2015): In vitro antimicrobial activity of five Egyptian plant species. Journal of Applied Pharmaceutical Science, 5, 045-049.

  93. Mortada M. El-Sayed, Maher M. El-Hashash, Heba R. Mohamed and Ezzat E. Abdel-Lateef (2015). Phytochemical Investigation and In vitro Antioxidant Activity of Different Leaf Extracts of Salix mucronata Thunb. J. Appl. Pharm. Sci., 5 (12): 80-85.

  94. Eman A. El-Wakil; Mortada M. El-Sayed; Ezzat E. Abdel-Lateef (2015). GC-MS Investigation of Essential Oil and Antioxidant Activity of Egyptian White Onion (Allium cepa L.). Int. J. Pharm. Sci. Res. 6(3): 537-543.

  95. Ghareeb, M.A., Refahy, L.A., Saad, A.M., Osman, N.S., El-Shazly, M.A., Mohamed, A.S. (2015): Cytotoxic screening of three Egyptian plants using brine shrimp lethality test. International Journal of Pharmacy and Pharmaceutical Sciences, 7, 507-509.

  96. El-Sayed S. Abdel-Hameed, Salih A. Bazaid and Abdel Nasser A. Sabra (2015): Total phenolic, in vitro antioxidant activity and safety assessment (Acute, sub-chronic and chronic toxicity) of industrial Taif rose water by-product in mice. Der Pharmacia Lettre, 7 (2):251-259.

  97. AbdEl-Gawad M.M , El-Nahas H.A , Osman N.S (2015): Molluscicidal activity of steroidal saponins isolated from Agave angustifolia. Global Journal of Pharmacology 9 (2): 138-143.

  98. Abdalla M.Ibrahim , Mahfouz M.AbdEl-Gawad , Hanan A.El-Nahas and Nadia S.Osman (2015) : Studies on the molluscicidal activity of Agave angustifolia and Pittosporum topira on Schistosomiasis transmitting snails.  J.Egypt.Soc.Parasitol.(JESP) , 45(1): 133-141.

  99. Hamed M, El-Amin S, Abdel-Fattah AS (2015): Effect of some environmental conditions on the molluscicidal activity of Yucca filamentosa "marginata" and Cestrum purpureum. Pharmacology online,  2: 142-147.

  100. Hamed M, El-Amin S, Abdel-Fattah AS (2015): Molluscicidal Activity of Some Constituents Isolated from "Cestrum purpureum". Pharmacology online, 2, 59-72.

  101. Hamed M, El-Amin S, Abdel-Fattah AS (2015): Isolation and identification of some compounds from molluscicidaly active plant Yucca filamentosa "marginata". Pharmacology online, 1: 19-30.

  102. Manal M. Hamed, Mona A. Mohamed, Magda T. Ibrahim (2015): Phytochemical Investigation and Cytotoxic Characterization of Bioactive Constituents from Conyza dioscoridis International Journal of Pharmacognosy and Phytochemical Research, 7(5); 948-955.

  103. Mona Mohamed1, Olov Sterner, Karl-Erik Bergquistt (2015): Phytochemical and Cytotoxic Studies on Calendula Officinalis. Life Science Journal; 12(1), 194-201.

  104. Rabab A. El Dib, Jacqueline Eskander, Mona A. Mohamed, Nermine M. Mohammed (2015): Two new triterpenoid estersaponins and biological activities of Pittosporum tobira ‘Variegata’ (Thunb.) W. T. Aiton leaves. Fitoterapia 106, 272–279.

  105. M.M. Hamed, M.A. Mohamed, L.A. Refai, O.A. Hammam, E.G. El-Ahwany, F.S. El-din, and H.I. Hassanein, The Active Constituents of Pelargonium zonale Induced Cytotoxicity in Human Hepatoma Cell Line HepG2. International Journal of Pharmaceutical Applications, l6 (1): 10-19 (2015).

  106. EL-Deeb A.E., I.M. Abdel-Aleem, S.M. El-Amin, L.A. Refahy, M.A. El-Shazly (2015): Active cytotoxic compounds and essential oil from Bougainvillea alba. Journal of Natural Remedies, 15 (1): 49-57.

  107. Hamed M. M., Refahy L. A.and Abdel-aziz M. S. (2015) Evaluation of Antimicrobial Activity of Some Compounds Isolated from Rhamnus cathartica L, Oriental Journal of Chemistry 31 (2): 1133-1140.

  108. Refahy LA, Farghaly TA, Abdel-Azia M and Mohamed TS. (2015):  Antimicrobial, Antioxidant and Cytotoxic Potential of Caesalpinia pulcherrima Flower.  Global Journal of Pharmacology 9 (2): 150-158.

  109. Eman A. El-Wakil, El-Sayed S. Abdel-Hameed, Mortada M. El-Sayed and Ezzat E. Abdel-Lateef (2015): Identification of the chemical composition of the methanolic extract of Salix tetrasperma Roxb. using LC-ESI-MS and evaluation its potential as antioxidant agent. Der Pharma Chemica, 7(2):168-177.

  110. Salem, M.S., Guirguis, D.B., El-Helw, E.A.E., Ghareeb, M.A., Derbala, H.A.Y. (2014): Antioxidant activity of heterocyclic compounds derived from 4-(4-Acetamidophenyl)-4-oxobut-2-enoic acid. International Journal of Science and Research, 3, 1274-1282.

  111. SeifHYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/?term=Seif%20el-Din%20SH%5BAuthor%5D&cauthor=true&cauthor_uid=24047470" el-Din SH, El-HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/?term=El-Lakkany%20NM%5BAuthor%5D&cauthor=true&cauthor_uid=24047470"LakkanyHYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/?term=El-Lakkany%20NM%5BAuthor%5D&cauthor=true&cauthor_uid=24047470" NM, Mohamed MA, Hamed MM, Sterner O, Botros SS (2014): Potential effect of the medicinal plants Calotropis procera, Ficus elastica and Zingiber officinale against Schistosoma mansoni in mice. Pharmaceutical Biology, 52(2):144-50.

  112. Mahfouz M. Abdel-Gawad; Maher M. Abdel-Aziz; Mortada M. El-Sayed; Eman A. El-Wakil; Ezzat E. Abdel-Lateef (2014). Chromatographic Isolation of Allium cepa (ssp. red onion) and its Cytotoxic Activity against Human Liver Carcinoma Cell Lines (HepG2). Int. J. pharm. Pharma. Sci. 6 (8): 108-111.

  113. Mahfouz Abdel-Gawad; Maher Abdel-Aziz; Mortada El-Sayed; Eman El-Wakil; Ezzat Abdel-Lateef (2014). In vitro Antioxidant, Total Phenolic and Flavonoid Contents of Six Allium Species Growing in Egypt. J. Microbiol. Biotech. Food Sci. 3 (4): 343-346.

  114. Ghareeb M.A., Shoeb, H.A., Madkour, H.M.F., Refahy, L.A., Mohamed, M.A., Saad, A.M. (2014): Antioxidant and cytotoxic activities of flavonoidal compounds from Gmelina arborea (Roxb.). Global Journal of Pharmacology, 8, 87-97.

  115. Mortada M. El-Sayed, Hanan, A. El-Nahas and El-Wakil E. A, (2014).           Molluscicidal Properties and Chemical Constituents of Euphorbia peploides. Australin J. of Basic and Applied Sciences, 8(3), 541-548.

  116. Ghareeb, M.A., Shoeb, H.S., Madkour, H.M.F., Refaey, L.A., Mohamed, M.A., Saad, A.M. (2014): Antioxidant and cytotoxic activities of Tectona grandis Linn leaves. International Journal of Phytopharmacology, 5, 143-157.

  117. Shoeb H.A., Madkour, H.M.F, Refahy, L.A., Mona A. Mohamed, M.A., Saad, A.M., Ghareeb, M.A., (2014): Antioxidant and cytotoxic activities of Gmelina arborea (ROXB.) leaves. British Journal of Pharmaceutical Research, 4, 125-144. 

  118. Laila A. Refahy and Amal M. Saad (2014): Characterization of Chemical Constituents, Reducing Power and Anticancer Activities of Terminalia bellerica Roxb Academic Journal of Cancer Research 7 (2): 117-125.

  119. William S, Saad A, Abdou A, Refahy L, Sabra A, Sterner O and Botros S. (2014): Potential Antischistosomal Activities of Some Egyptian Native Plants Using Schistosoma mansoni Worm Killing Assay. Global Journal of Pharmacology 8 (2): 237-244.

  120. Mahmood Salman; El-Sayed S. Abdel-Hameed; Salih A. Bazaid; Musbah G. Al-Shamrani and Hanaa F. Mohamed (2014): Liquid chromatography-mass spectrometry (LC-MS) method for the determination of sugars in fresh pomegranate fruit juices. Der Pharma Chemica,  6(5):320-333.

  121. Mahmood Salman; El-Sayed S. Abdel-Hameed; Salih A. Bazaid; and Musbah G. Al-Shamrani (2014): Atomic absorption spectrometry and flame photometry for determination of minerals elements in fresh pomegranate fruit juice. Der Pharma Chemica, 6(6):149-155.

  122. Afaf A. Abdel-Hady, Hanan A. El-Nahas, Salwa K. El Nabarawy and Heba A. Abdel Raouf. (2014): Evaluation of the Antioxidant Activity and the Acute Oral Toxicity of Three Plant Extracts on Albino Mice. Middle East Journal of Applied Sciences, 4(2): 207-216.

  123. Hamed, M. M., El-Amin, S. M. A. Refahy, L. A., Soliman, E. A., Mansour, W. A., Abu Taleb, H. M. and Morsi, E. A. (2015). Anticancer and antiviral estimation of three ulmus pravifolia extracts and their chemical constituents. Oriental journal of chemistry.31 (3)1621-1634.

  124. Hanan A El-Nahas, Salwa K El Nabarawy , Afaf A Abdel-Hady, Ahmed M Abdel-Hady, Heba A Abdel Raouf. (2014): Effect of methanol extracts of three dietary plants growing in Egypt on mice fed with high fat diet. Journal of Applied Pharmaceutical Science Vol. 4 (05), pp. 104-111.

  125. El-Sayed S. Abdel-Hameed; Salih A. Bazaid; Othman Al Zahrani; Yasser El-Halmouch; Mortada M. El-Sayed; Eman  El-Wakil (2014): Chemical composition of volatile components, antimicrobial and  anticancer activity of n-hexane extract and essential oil from Trachyspermum ammi L. seeds. Oriental Journal of Chemistry Vol. 30(4), 1653-1662.

  126. Mohamed M. Shohayeb, El-Sayed S. Abdel-Hameed, Salih A. Bazaid; Ibrahim M. (2014): Antibacterial and Antifungal Activity of Rosa damascena MILL. Essential Oil, Different Extracts of Rose Petals. Global Journal of Pharmacology 8 (1): 1-7.

  127. El-Sayed S. Abdel-Hameed; Salih A. Bazaid, Mohamed M. Shohayeb (2014): RP-HPLC-UV–ESI-MS phytochemical analysis of fruits of Conocarpus erectus L. Chemical Papers. 68 (10) 1358–1367.

  128. El-Sayed S. Abdel-Hameed; Mohamed A. Nagaty;  Mahmood S. Salman, Salih A. Bazaid (2014): Phytochemicals, nutritionals and antioxidant properties of two prickly pear cactus cultivars (Opuntia ficus indica Mill.) growing in Taif, KSA. Food Chemistry 160 (2014) 31-38.

  129. Heba A. Hagag, Salih A. Bazaid, El-Sayed S. Abdel-Hameed, Mahmood Salman (2014). Cytogenetic, cytotoxic and GC–MS studies on concrete and absolute oils from Taif rose, Saudi Arabia. Cytotechnology, 66(6):913-23.

  130. Ghareeb M.A., Shoeb H.A., Madkour H.M.F., Refahy L.A., Mohamed M.A., Saad A.M. (2013). Radical scavenging potential and cytotoxic activity of phenolic compounds from Tectona grandis (Linn.). Global Journal of Pharmacology. 7(4): 486-497.

  131. El-Sayed M., El-Nahas H., Abdel-Hameed E., El-Waked E. (2013). Investigation and antioxidant of phenolic compounds of the leaves of Gleditsia tricanthos. Int J Pharm and Pharmaceutical Science. 5(2): 172-177.

  132. El-Sayed M., Abdel-Aziz M., Abdel-Gwad M., Abdel-Hameed E., Sabry W., Abdel-Lateef E. (2013). Chemical constituents and cytotoxic activity of Cassia glauca Linn Leaves. Life Science Journal. 10(3): 1617-1625.

  133. Mohamed M., Abdou A., Saad A. and Ibrahim M. (2013). Cytotoxic activity of Buddleja asiatica. Journal of life Science. 10(1): 2773-2777.

  134. Abdallah H., Mohamed M., Abdou A., Hamed M., Abdel-Naim A. and Ashour O. (2013). Protective effect of Centaurea Pallescens Del. against CCl4-induced injury on a human hepatoma cell line (Huh7), Medicinal Chemistry Research. 22(4): 5700-5706.

  135. Abdou A., Abdallah H., Mohamed M., Fawzy G. and Abdel-Naim A. (2013). A new Anti-inflammatory triterperpene saponin isolated from Anabasis setifera. Archive of Pharmacal Research. 36(6): 715-722.

  136. Seif el-Din S., El-Lakkany N., Mohamed M., Hamed M., Sterner O., Botros S. (2013). Potential effect of the medicinal plants Calotropis procera, Ficus elastica and Zingiber officinale against Schistosoma mansoni in mice. Pharmaceutical Biology, 1-7.

  137. Abdel-Hameed E., Bazaid S., Shohayeb M., El-Sayed M., El-Wakel E. (2012). Phytochemical studies and evaluation of antioxidant, anticancer and antimicrobial properties of Conocarpus erectus growing in Taif, Saudi Arabia. European Journal of Medicinal plants. 2(2): 93-112.

  138. Mohamed M., Abdou A., Hamed M. and Saad A. (2012). Characterization of Bioactive Phytochemical from the leaves of Vitex trifolia. International Journal of Pharmaceutical applications. 3(4): 419-428.

  139. Mahmoud M., EL-Amin S., Refahy L. and Saad A. (2012). Possible antischistosomal and antinenoceptive activity of different extracts of Glycerrhiza glabra. International Journal of Pharmaceutical Applications. 3 (2): 360-369.

  140. Mona A. Mohamed, Allia M. Abdou, Manal M. Hamed and Amal M. Saad. (2012). Characterization of bioactive phytochemical from the leaves of Vitex trifolia. International Journal of Pharmaceutical Applications. 3 (4): 419-428.

  141. El-Sayed M., Abdel-Hady A., Sedra N., Mouhamed M., El-Wakel E., Ghareeb M. (2011). Effect of Ficus sycomorus and Azadirachta indica extracts on liver state mice infected with Schistosoma mansoni. Journal of Egyptian Society of Parasitology. 41(1): 1-12.

  142. El-Sayed M., Mohamed M., El-Nahas H., El-Toumy S., El-Wakel E., Ghareeb M., (2011). Chemical constituents, antischistosomal and antioxidant activities of methanolic extracts of Azadirachta indica. Egyptian Journal of Chemistry, 54(1): 99-113.

  143. Haggag E., Kamel A., Abdel-Haddy M., El-Sayed M., El-Wakel E., Abdel-Hameed S. (2011). Antioxidant and cytotoxic activity of polyphenolic compounds isolated from the leaves of Leucenia leucocephala. Pharmaceutical Biology. 49(11): 1103-1113.

  144. El-Sayed M., El-Hashash M., Mohamed M., Korany T. (2011). Cytotoxic activity of Vitex trifolia purpurea extracts. Journal of Egyptian Society of Parasitology, 41(2): 409-416.

  145. El-Sayed M., Abo-Sedra S., El-Nahas H., Abdel-Gwad M., Abdel-Aziz M., Sabry W., Abdel-Lateef E. (2011). Evaluation of antioxidant and antimicrobial activities of certain Cassia species. Australian Journal of Basic and Applied Sciences. 5(9): 344-352.

  146. Refahy L. (2011). Study on Flavonoids and Triterpenoids Content of some Euphorbiaceae Plants. Journal of Life Sciences. 5: 100-107.

  147. Mohamed M., Hamed M., Abdou A., Ahmed W. and Saad A. (2011). Antioxidant and cytotoxic flavonols from Calotropis procera. Zeitschrift für Naturforschung. 66c: 547-554.

  148. Mohamed M., Hamed M., Abdou A., Ahmed W. and Saad A. (2011). Antioxidant and Cytotoxic Constituents from Wisteria sinensis. Molecules. 16 (5): 4020-4030.

  149. Hassanein H., El-Ahwany E., Salah F., Hammam O., Refai L and Hamed M. (2011). Extracts of Five Medicinal Herbs Induced Cytotoxicity in Both Hepatoma and Myeloma Cell Lines. Journal of Cancer Sciences and Therapy. 3(10): 239-243.